anti-hnf4α  (Cell Signaling Technology Inc)

Journal: Cancer Cytopathology

Article Title: Hepatocyte nuclear factor 4 alpha immunocytochemistry: A useful marker for detecting endocervical glandular lesions in alcohol‐fixed smears

doi: 10.1002/cncy.70034

Figure Lengend Snippet: Assessment of the diagnostic potential of HNF4α expression for detecting EGLs. (A) Accuracy of morphology‐based cytological analysis for detecting EGLs by smears. (B) Accuracy of HNF4α immunohistochemistry for detecting EGLs using FFPE issue specimens. (C) Accuracy of HNF4α immunocytochemistry for detecting EGLs by smears. AUC indicates area under the curve; EGL, endocervical glandular lesion; FFPE, formalin‐fixed paraffin‐embedded; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha.

Article Snippet: Then, the slides were incubated with anti‐human HNF4α mouse monoclonal antibody (#H1415, recognizing the C‐terminal isoform, 1:1000; Perseus Proteomics, Tokyo, Japan) at 4°C overnight.

Techniques: Diagnostic Assay, Expressing, Immunohistochemistry, Immunocytochemistry, Formalin-fixed Paraffin-Embedded

Journal: Cancer Cytopathology

Article Title: Hepatocyte nuclear factor 4 alpha immunocytochemistry: A useful marker for detecting endocervical glandular lesions in alcohol‐fixed smears

doi: 10.1002/cncy.70034

Figure Lengend Snippet: Alcohol‐fixed cytological smears of EGLs and HNF4α immunocytochemical staining. (A,B) Case of cytologically diagnosed adenocarcinoma, histologically confirmed as HPVA ECA, showing a large cluster with nuclei with intense and diffuse HNF4α expression (arrowhead) and normal squamous cells without HNF4α staining (arrow). (C,D) Case of cytologically diagnosed AGC, histologically confirmed as NHPVA ECA, showing slightly nuclear atypia with HNF4α staining (arrowhead) and normal glands without HNF4α staining (arrow). (E,F) Case of cytologically diagnosed AGC, histologically confirmed as LEGH, showing yellowish mucin with HNF4α staining (arrowhead) and normal glands without HNF4α staining (arrow). (A,C,E) Papanicolaou staining, 200× magnification. (B,D,F) HNF4α immunocytochemical staining, 200× magnification. ECA indicates endocervical adenocarcinoma; EGL, endocervical glandular lesions; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha; HPVA, human papillomavirus–associated; LEGH, lobular endocervical glandular hyperplasia; NHPVA, nonhuman papillomavirus–associated.

Article Snippet: Then, the slides were incubated with anti‐human HNF4α mouse monoclonal antibody (#H1415, recognizing the C‐terminal isoform, 1:1000; Perseus Proteomics, Tokyo, Japan) at 4°C overnight.

Techniques: Staining, Expressing

Journal: Cancer Cytopathology

Article Title: Hepatocyte nuclear factor 4 alpha immunocytochemistry: A useful marker for detecting endocervical glandular lesions in alcohol‐fixed smears

doi: 10.1002/cncy.70034

Figure Lengend Snippet: HNF4α immunocytochemical staining in smears of EGLs. (A,B) Cytologically and histologically diagnosed SCC lacking HNF4α expression. (C,D) Cytologically diagnosed ASC‐US and histologically confirmed as HPVA ECA showing a cluster with mild nuclear atypia with diffuse HNF4α expression on an inflammatory background. (A,C) Papanicolaou staining: (A) 200× and (C) 400× magnification. (B,D) HNF4α immunocytochemical staining (B) 200× and (D) 400× magnification. ASC‐US indicates atypical squamous cells of undetermined significance; ECA, endocervical adenocarcinoma; EGL, endocervical glandular lesion; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha; HPVA, human papillomavirus–associated; SCC, squamous cell carcinoma.

Article Snippet: Then, the slides were incubated with anti‐human HNF4α mouse monoclonal antibody (#H1415, recognizing the C‐terminal isoform, 1:1000; Perseus Proteomics, Tokyo, Japan) at 4°C overnight.

Techniques: Staining, Expressing

Journal: Cancer Cytopathology

Article Title: Hepatocyte nuclear factor 4 alpha immunocytochemistry: A useful marker for detecting endocervical glandular lesions in alcohol‐fixed smears

doi: 10.1002/cncy.70034

Figure Lengend Snippet: HNF4α expression in paired FFPE surgical tissue specimens and alcohol‐fixed smears. (A,B) HPVA ECA in FFPE surgical tissue specimen with HNF4α expression in ECA glands (arrowhead) and without HNF4α expression in normal glands (arrow). (C,D) Paired alcohol‐fixed smear showing HNF4α expression in a large cluster consisting of atypical gland cells (arrowhead) and no HNF4α expression in nonatypical glands (arrow). (A) Hematoxylin–eosin staining, 100× magnification. (B) HNF4α immunohistochemical staining, 100× magnification. (C) Papanicolaou staining, 40× magnification. (D) HNF4α immunocytochemical staining, 40× magnification. ECA indicates endocervical adenocarcinoma; FFPE, formalin‐fixed paraffin‐embedded; HNF, hepatocyte nuclear factor; HNF4α, hepatocyte nuclear factor 4 alpha; HPVA, human papillomavirus–associated.

Article Snippet: Then, the slides were incubated with anti‐human HNF4α mouse monoclonal antibody (#H1415, recognizing the C‐terminal isoform, 1:1000; Perseus Proteomics, Tokyo, Japan) at 4°C overnight.

Techniques: Expressing, Staining, Immunohistochemical staining, Formalin-fixed Paraffin-Embedded

Journal: Stem cell research & therapy

Article Title: Hepatic progenitor cells reprogrammed from mouse fibroblasts repopulate hepatocytes in Wilson's disease mice.

doi: 10.1186/s13287-025-04253-1

Figure Lengend Snippet: Fig. 1 Coexpression of Foxa3 and Hnf4α reprogrammed mouse fibroblasts into iHPCs. (A) Constructs of the control-lentiviral vector and the overexpres sion-lentiviral vector carrying EF1α-promoter-driven Foxa3 and CMV-promoter-driven Hnf4α. (B) Representative phase-contrast morphology of control fibroblasts and iHPCs on Days 0, 2, 7, and 14 after lentivirus infection. Scale bars, 100 μm. (C) Immunofluorescence staining Flag and DAPI in control fibroblasts and iHPCs. Scale bars, 50 μm. (D) Immunofluorescence staining and flow cytometry were used to analyse the percentages of Flag-positive control fibroblasts and iHPCs. (E) Real-time PCR was used to determine the relative expression of Foxa3 and Hnf4α in control fibroblasts and iHPCs. (F) Western blot analysis showing the relative protein levels of Foxa3, Hnf4α, Alb, Cdh1, and β-Actin in control fibroblasts and iHPCs. β-Actin served as a load ing control. (G) Giemsa staining (scale bars, 50 μm), PAS staining (scale bars, 25 μm), and phase-contrast morphology of control fibroblasts and iHPCs at serial passages (scale bars, 100 μm)

Article Snippet: After being blocked with TBST buffer containing 5% nonfat dry milk, the membranes were incubated with one of the following primary antibodies: Hnf4α (Cell Signaling Technology, Danvers, MA, 1:1000), albumin (Alb, R&D Systems, Minneapolis, MN, 1:1000), E-cadherin (Cdh1, Abcam, Cambridge, UK, 1:1000), Foxa3 (Abcam, 1:1000), or β-Actin (Abcam, 1:1000).

Techniques: Construct, Control, Plasmid Preparation, Infection, Immunofluorescence, Staining, Flow Cytometry, Positive Control, Real-time Polymerase Chain Reaction, Expressing, Western Blot